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Abstract of EP0299790A C-terminal alpha -amidating enzyme of Xenopus laevis and precursor thereof produced by a recombinant DNA technique; a DNA coding for the enzyme or precursor thereof; a plasmid containing the DNA; a host organism transformed with the plasmid; a process for production of the enzyme using the transformant; and a process for production of a C-terminal alpha -amidated peptide using the enzyme. MIZUNO et al.: "Cloning and sequence of c DNA encoding a peptide C-terminal alpha-amidating enzyme from Xenopus laevis skin" BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 3, 15th February 1988, pages 1275-1281, Academic Press, Inc.; K.

Ohsuye, Kazuhiro (1-6-2 Yamatedai, Ibaraki-shi, Osaka, JP) Kitano, Katsuhiko (2-13-23-402 Ishibashi, Ikeda-shi, Osaka, JP) Tanaka, Shoji (4-9-1 Shiomi Cho, Ashiya-shi, Hyogo, JP) Matsuo, Hisayuki (5-15-141Onoharahigashi 5-Chome, Minoo shi, Osaka, JP) Mizuno, Kensaku (450 Ooaza Kihara Kiyotake-cho, Miyazaki-gun, Miyazaki, JP) BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 3, 30th June 1986, pages 984-991, Academic Press, Inc.; K.

and precursor thereof produced by a recombinant DNA technique; a DNA coding for the enzyme or precursor thereof; a plasmid containing the DNA; a host organism transformed with the plasmid; a process for production of the enzyme using the transformant; and a process for production of a C-terminal α-amidated peptide using the enzyme. A process according to claim 2 wherein the prepro- C-terminal α-amidating enzyme has amino acid sequence (I) and wherein A represents the amino acid sequence (II) and B represents the amino acid sequence (III).7.

A process according to claim 17, wherein the DNA has nucleotide sequence (XIV) where E represents the nucleotide sequence (XVI), where L represents GCT, M represents ACG, and N represents GCA, and F represents the nucleotide sequence (XVII).19.A process according to claim 17, wherein the DNA has nucleotide sequence (XIV) where E represents the nucleotide sequence (XVI), where L represents GCT, M represents ACG, and N represents GCA, and F represents the nucleotide sequence (XX).22.A process according to claim 17, wherein the DNA has nucleotide sequence (XIV) where E represents the nucleotide sequence (XVI), where L represents GCT, M represents ACG, and N represents GCA, and F is absent.23.A process according to claim 6 wherein the C-terminal α-amidating enzyme has amino acid sequence (IV) and wherein C represent the amino acid sequence (IV), X represents Ala, Y represents Thr, and Z represents Ala, and D represents the amino acid sequence (X).11.A process according to claim 6 wherein the C-terminal α-amidating enzyme has amino acid sequence (IV) and wherein C represents the amino acid sequence (VI), X represents Ala, Y represents Thr, and Z represents Ala, and D is absent.12.

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